Hotspot

ycf1

SSC · 128963-134602 · 5640 bp

Back to Chaenomeles

Pi 0.000355

MarkerSeek score 43.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5640
variable_sites: 3
pi: 0.000355
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGAACAGTTTGAATTGCA	ACACTCTCAAGCATAAATGAAGC	40.0	39.1	56.0	57.4	307 bp	1.0	40.5
ycf1_p2	TTGGGAACAGTTTGAATTGCA	ACACTCTCAAGCATAAATGAAGC	38.1	39.1	56.8	57.4	308 bp	1.0	40.5
ycf1_p3	GTTGGGAACAGTTTGAATTGCA	ACACTCTCAAGCATAAATGAAGC	40.9	39.1	58.2	57.4	309 bp	1.0	40.5
ycf1_p4	TGTTGGGAACAGTTTGAATTGC	ACACTCTCAAGCATAAATGAAGC	40.9	39.1	58.2	57.4	310 bp	1.0	40.5
ycf1_p5	TGGGAACAGTTTGAATTGCA	CACTCTCAAGCATAAATGAAGCT	40.0	39.1	56.0	57.2	306 bp	1.0	40.5