Hotspot

trnL-UAG

SSC · 119219-119298 · 80 bp

Back to Chaenomeles

Pi 0.00625

MarkerSeek score 42.80

Primer available yes

Label trnL-UAG

Feature Metadata

label_name: trnL-UAG
feature_type: tRNA
region: SSC
length_bp: 80
variable_sites: 1
pi: 0.00625
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG_p1	CCTTCAAGCTCGACGATTGA	GGGCGTCCCTCAATTATAGG	50.0	55.0	58.0	57.5	204-264 bp	1.0	76.5
trnL-UAG_p2	TCCTTCAAGCTCGACGATTG	GGGCGTCCCTCAATTATAGG	50.0	55.0	58.0	57.5	205-265 bp	1.0	76.5
trnL-UAG_p3	CTCCTTCAAGCTCGACGATT	GGGCGTCCCTCAATTATAGG	50.0	55.0	57.7	57.5	206-266 bp	1.0	74.3
trnL-UAG_p4	TAACTCCTTCAAGCTCGACG	GGGCGTCCCTCAATTATAGG	50.0	55.0	57.4	57.5	209-269 bp	1.0	71.5
trnL-UAG_p5	ACTCCTTCAAGCTCGACGAT	GGGCGTCCCTCAATTATAGG	50.0	55.0	58.8	57.5	207-267 bp	1.0	70.0