Hotspot

trnC-GCA

LSC · 29016-29086 · 71 bp

Back to Chaenomeles

Pi 0.0141

MarkerSeek score 48.30

Primer available yes

Label trnC-GCA

Feature Metadata

label_name: trnC-GCA
feature_type: tRNA
region: LSC
length_bp: 71
variable_sites: 2
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA_p1	GTGGATCCAACCCAACCAAA	TTTCCTCCACTTGCTTCTGC	50.0	50.0	58.3	58.4	273 bp	1.0	75.1
trnC-GCA_p2	TGGATCCAACCCAACCAAAA	TTTCCTCCACTTGCTTCTGC	45.0	50.0	57.5	58.4	272 bp	1.0	73.6
trnC-GCA_p3	GTGGATCCAACCCAACCAAA	CCACTTGCTTCTGCTGGAAA	50.0	50.0	58.3	58.7	267 bp	1.0	72.9
trnC-GCA_p4	TGGATCCAACCCAACCAAAA	CCACTTGCTTCTGCTGGAAA	45.0	50.0	57.5	58.7	266 bp	1.0	71.3
trnC-GCA_p5	GTGGATCCAACCCAACCAAA	CCACTTGCTTCTGCTGGAA	50.0	52.6	58.3	58.0	267 bp	1.0	70.1