Hotspot

psaA

LSC · 42278-44530 · 2253 bp

Back to Chaenomeles

Pi 0.000518

MarkerSeek score 48.20

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: LSC
length_bp: 2253
variable_sites: 2
pi: 0.000518
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	AGCTAAGCCTTGGCTAAACC	GCGGGTCTCTTTGTATGTGT	50.0	50.0	57.9	57.9	2359 bp	1.0	78.3
psaA_p2	GCTAAGCCTTGGCTAAACCT	GCGGGTCTCTTTGTATGTGT	50.0	50.0	57.9	57.9	2358 bp	1.0	78.3
psaA_p3	AATACGACGAGTAGTGGGGT	GCGGGTCTCTTTGTATGTGT	50.0	50.0	57.9	57.9	2383 bp	1.0	78.2
psaA_p4	GTAGTGGGGTCCTGAGCTAA	GCGGGTCTCTTTGTATGTGT	55.0	50.0	58.1	57.9	2373 bp	1.0	78.2
psaA_p5	GCTAAGCCTTGGCTAAACCT	TTGGCGGGTCTCTTTGTATG	50.0	50.0	57.9	57.9	2361 bp	1.0	78.2