Hotspot

ycf3-trnS

LSC · 47196-47810 · 615 bp

Back to Chaenomeles

Pi 0

MarkerSeek score 49.70

Primer available yes

Label ycf3-trnS

Feature Metadata

label_name: ycf3-trnS
feature_type: igs
region: LSC
length_bp: 615
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS_p1	TGCCTCTTTTTCTCCCGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	806-808 bp	1.0	77.5
ycf3-trnS_p2	GCCTCTTTTTCTCCCGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	805-807 bp	1.0	77.5
ycf3-trnS_p3	TGCCTCTTTTTCTCCCGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	756-758 bp	1.0	77.2
ycf3-trnS_p4	GCCTCTTTTTCTCCCGAAGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	755-757 bp	1.0	77.2
ycf3-trnS_p5	TGCCTCTTTTTCTCCCGAAG	AGTTCCAATGCTACGCCTTG	50.0	50.0	57.8	58.5	758-760 bp	1.0	74.4