Hotspot

trnR-UCU-atpA

LSC · 10129-10797 · 669 bp

Back to Chaenomeles

Pi 0.00797

MarkerSeek score 49.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 669
variable_sites: 8
pi: 0.00797
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	832-838 bp	1.0	67.8
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	831-837 bp	1.0	67.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	771-777 bp	1.0	67.1
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	AGACATTTACCGACGAAGCG	47.6	50.0	56.9	58.4	806-812 bp	1.0	64.1
trnR-UCU-atpA_p5	TGGAATGAAAAGCGTCCATT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	832-838 bp	1.0	63.7