Hotspot

psbK-psbI

LSC · 7866-8117 · 252 bp

Back to Chaenomeles

Pi 0.00265
MarkerSeek score 33.60
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
252
variable_sites
1
pi
0.00265
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG TCTTCACGTCCAGGATTACG 50.0 50.0 57.9 57.1 389 bp 1.0 72.3
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT TCTTCACGTCCAGGATTACG 45.0 50.0 58.3 57.1 386 bp 1.0 70.3
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT TCTTCACGTCCAGGATTACG 45.0 50.0 58.3 57.1 387 bp 1.0 70.3
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT TCTTCACGTCCAGGATTACG 45.0 50.0 58.3 57.1 388 bp 1.0 70.3
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG TTCTTCACGTCCAGGATTACG 50.0 47.6 57.9 57.8 390 bp 1.0 69.9