Hotspot

rpoC1

LSC · 21013-23838 · 2826 bp

Back to Ceropegia

Pi 0.00212

MarkerSeek score 58.10

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2826
variable_sites: 12
pi: 0.00212
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	ACTATAATCCCGGAGAGGCC	GAAGGAATAGCAGGGAAGGC	55.0	55.0	58.1	58.0	2897-2911 bp	1.0	80.0
rpoC1_p2	TTTCTTGACTCGATTCGCGT	GAAGGAATAGCAGGGAAGGC	45.0	55.0	57.9	58.0	2929-2943 bp	1.0	80.0
rpoC1_p3	TATAATCCCGGAGAGGCCTG	GAAGGAATAGCAGGGAAGGC	55.0	55.0	58.1	58.0	2895-2909 bp	1.0	80.0
rpoC1_p4	ACTATAATCCCGGAGAGGCC	AGCAGGGAAGGCTTAGAAGA	55.0	50.0	58.1	58.0	2889-2903 bp	1.0	79.9
rpoC1_p5	TTTCTTGACTCGATTCGCGT	AGCAGGGAAGGCTTAGAAGA	45.0	50.0	57.9	58.0	2921-2935 bp	1.0	79.9