Hotspot

ndhA

SSC · 126586-128788 · 2203 bp

Back to Ceropegia

Pi 0.00719

MarkerSeek score 38.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2203
variable_sites: 31
pi: 0.00719
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGACAGGGTAATCGCGAAAC	ACGAATCGGTGAAATGGGAG	50.0	50.0	58.0	58.0	2779-2806 bp	1.0	80.7
ndhA_p2	AGACAGGGTAATCGCGAAAC	GTTTATCGGGCCCAATGCTA	50.0	50.0	58.0	58.0	2915-2942 bp	1.0	80.7
ndhA_p3	AGACAGGGTAATCGCGAAAC	TTTATCGGGCCCAATGCTAC	50.0	50.0	58.0	58.0	2914-2941 bp	1.0	80.7
ndhA_p4	AGACAGGGTAATCGCGAAAC	TTTTAGAACGGGTTGAGGGC	50.0	50.0	58.0	58.1	2972-2999 bp	1.0	80.1
ndhA_p5	AGACAGGGTAATCGCGAAAC	ATTCGCGAAGAAGAAGCACT	50.0	45.0	58.0	57.9	2943 bp	0.75	69.5