Hotspot

ndhG-ndhI

SSC · 125463-126003 · 541 bp

Back to Ceropegia

Pi 0.0142

MarkerSeek score 47.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 541
variable_sites: 15
pi: 0.0142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACCTCCTAAGATCAGACCCG	GTTTCGCGATTACCCTGTCT	55.0	50.0	57.9	58.0	1032-1069 bp	1.0	82.1
ndhG-ndhI_p2	ATCAGACCCGATCCCAGAAA	GTTTCGCGATTACCCTGTCT	50.0	50.0	58.1	58.0	1022-1059 bp	1.0	81.8
ndhG-ndhI_p3	ACAAACCACCGAAGTAACCC	GTTTCGCGATTACCCTGTCT	50.0	50.0	58.0	58.0	1275-1312 bp	0.75	71.9
ndhG-ndhI_p4	ACAAACCACCGAAGTAACCC	CACAATTTCGAATTCGCCCC	50.0	50.0	58.0	58.1	881-918 bp	0.75	71.9
ndhG-ndhI_p5	ACAAACCACCGAAGTAACCC	ACAAACAATACGAGCTGCCA	50.0	45.0	58.0	58.1	1310-1347 bp	0.75	71.0