Hotspot

rpl20-rps12

LSC · 70593-71464 · 872 bp

Back to Ceropegia

Pi 0.00229

MarkerSeek score 56.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 4
pi: 0.00229
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TAAGTTTTGAATGGGCCCCC	CTCAACGTCGAGGAACATGT	50.0	50.0	58.1	57.9	998-1009 bp	1.0	80.2
rpl20-rps12_p2	TAAGTTTTGAATGGGCCCCC	GAGGAACATGTACTCGGGTG	50.0	55.0	58.1	57.7	989-1000 bp	1.0	78.4
rpl20-rps12_p3	CGAGCTATATATCCCCGGCT	CTCAACGTCGAGGAACATGT	55.0	50.0	58.3	57.9	936-947 bp	1.0	78.3
rpl20-rps12_p4	TAAGTTTTGAATGGGCCCCC	AGGAACATGTACTCGGGTGT	50.0	50.0	58.1	58.4	988-999 bp	1.0	77.9
rpl20-rps12_p5	TCCCCGGCTAATTCTAGTCA	CTCAACGTCGAGGAACATGT	50.0	50.0	57.5	57.9	925-936 bp	1.0	77.0