Hotspot

atpB-rbcL

LSC · 56596-57343 · 748 bp

Back to Ceropegia

Pi 0.00491

MarkerSeek score 62.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 7
pi: 0.00491
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGCCCCAGGTTGTTTTT	AACACCGGCTTTGAATCCAA	45.0	45.0	58.0	58.3	866-868 bp	1.0	78.6
atpB-rbcL_p2	CTACATCTAGTACCGGGCCA	AACACCGGCTTTGAATCCAA	55.0	45.0	58.0	58.3	898-900 bp	1.0	78.5
atpB-rbcL_p3	GCGCAAAGGCTACATCTAGT	AACACCGGCTTTGAATCCAA	50.0	45.0	58.1	58.3	907-909 bp	1.0	78.1
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	AACACCGGCTTTGAATCCAA	55.0	45.0	58.1	58.3	837-839 bp	1.0	77.9
atpB-rbcL_p5	TCTAGTACCGGGCCAATGAT	AACACCGGCTTTGAATCCAA	50.0	45.0	57.9	58.3	893-895 bp	1.0	77.8