Hotspot

ndhC-trnV-UAC

LSC · 52196-53534 · 1339 bp

Back to Ceropegia

Pi 0.0138
MarkerSeek score 59.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1339
variable_sites
35
pi
0.0138
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGAATCGCGACCCTATTAGG TGAATCAGAGCACATGGAGC 55.0 50.0 58.0 58.0 2127-2145 bp 1.0 83.3
ndhC-trnV-UAC_p2 CGAATCGCGACCCTATTAGG GGTTGATCTAGGCCAGAAGC 55.0 55.0 58.0 58.0 2216-2234 bp 1.0 83.1
ndhC-trnV-UAC_p3 CCGGTAATTGTACAGGCTCC TGAATCAGAGCACATGGAGC 55.0 50.0 58.1 58.0 2293-2311 bp 1.0 82.9
ndhC-trnV-UAC_p4 CCTCCGGTAATTGTACAGGC TGAATCAGAGCACATGGAGC 55.0 50.0 58.1 58.0 2296-2314 bp 1.0 82.9
ndhC-trnV-UAC_p5 CGGTAATTGTACAGGCTCCC TGAATCAGAGCACATGGAGC 55.0 50.0 58.1 58.0 2292-2310 bp 1.0 82.9