Hotspot

rpoB-trnC-GCA

LSC · 27067-28354 · 1288 bp

Back to Ceropegia

Pi 0.00802

MarkerSeek score 51.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1288
variable_sites: 20
pi: 0.00802
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTGCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1342-1423 bp	1.0	82.3
rpoB-trnC-GCA_p2	GCAAACCCCGATCAATGAAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1410-1491 bp	1.0	80.6
rpoB-trnC-GCA_p3	ATTCCCTCATTTGCATCCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	1324-1405 bp	1.0	80.2
rpoB-trnC-GCA_p4	GCAAACCCCGATCAATGAAC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1392-1473 bp	1.0	78.5
rpoB-trnC-GCA_p5	ATTCCCTCATTTGCATCCCC	TTTGTTGATCAGGCGACACC	50.0	50.0	57.9	58.8	1361-1442 bp	1.0	76.8