Hotspot

trnP-UGG-psaJ

LSC · 75884-76368 · 485 bp

Back to Ceriops

Pi 0.0466

MarkerSeek score 79.50

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 32
pi: 0.0466
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	ACTGGTGCCACGGATAGATA	50.0	50.0	58.1	57.9	578-604 bp	1.0	86.7
trnP-UGG-psaJ_p2	ACCCGTGACATTTTGTACCC	TACTGGTGCCACGGATAGAT	50.0	50.0	58.1	57.9	579-605 bp	1.0	86.7
trnP-UGG-psaJ_p3	CCCGTGACATTTTGTACCCA	ACTGGTGCCACGGATAGATA	50.0	50.0	58.1	57.9	577-603 bp	1.0	86.7
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	TACTGGTGCCACGGATAGAT	50.0	50.0	58.1	57.9	578-604 bp	1.0	86.7
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	ACTGGTGCCACGGATAGATA	50.0	50.0	58.1	57.9	589-615 bp	1.0	86.5