Hotspot

accD-psaI

LSC · 67220-68097 · 878 bp

Back to Ceriops

Pi 0.0525

MarkerSeek score 80.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 878
variable_sites: 63
pi: 0.0525
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACGCCTACATTGCATTTGC	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	1129-1201 bp	1.0	88.3
accD-psaI_p2	AACGCCTACATTGCATTTGC	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	1135-1207 bp	1.0	87.6
accD-psaI_p3	AACGCCTACATTGCATTTGC	GTTTTTCGAGACCCCGCTAT	45.0	50.0	58.0	58.0	1643-1731 bp	1.0	87.5
accD-psaI_p4	AACGCCTACATTGCATTTGC	ATGTTTGAAGGAAGTCGCGT	45.0	45.0	58.0	58.1	1286-1358 bp	1.0	87.2
accD-psaI_p5	AACGCCTACATTGCATTTGC	TCGAGACCCCGCTATAAACT	45.0	50.0	58.0	57.9	1638-1726 bp	1.0	87.1