Hotspot

ndhC-trnV

LSC · 57696-59391 · 1696 bp

Back to Ceriops

Pi 0.0373

MarkerSeek score 72.20

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 1696
variable_sites: 74
pi: 0.0373
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TGCCCAGAAAATATCATATTCGT	GGTTCGAATCCGTATAGCCC	34.8	55.0	56.1	57.9	1465-1754 bp	1.0	48.0
ndhC-trnV_p2	TGCCCAGAAAATATCATATTCGT	CGGTTCGAATCCGTATAGCC	34.8	55.0	56.1	58.3	1466-1755 bp	1.0	47.7
ndhC-trnV_p3	TGCCCAGAAAATATCATATTCGT	CAGGTCTACGGTTCGAATCC	34.8	55.0	56.1	57.8	1474-1763 bp	1.0	47.6
ndhC-trnV_p4	CGCTTGATATTATTAGAAATGCCCA	GGTTCGAATCCGTATAGCCC	36.0	55.0	57.9	57.9	1484-1773 bp	1.0	47.6
ndhC-trnV_p5	ACGCTTGATATTATTAGAAATGCCC	GGTTCGAATCCGTATAGCCC	36.0	55.0	57.9	57.9	1485-1774 bp	1.0	47.6