Hotspot

ycf3-trnS-GGA

LSC · 51796-52259 · 464 bp

Back to Ceriops

Pi 0.0189

MarkerSeek score 71.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 13
pi: 0.0189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTTTCTCCCAAGGTTGTCGG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.0	58.1	617-723 bp	1.0	82.6
ycf3-trnS-GGA_p2	TTTTCTCCCAAGGTTGTCGG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.0	58.2	638-744 bp	1.0	82.0
ycf3-trnS-GGA_p3	TTTTCTCCCAAGGTTGTCGG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	588-694 bp	1.0	82.0
ycf3-trnS-GGA_p4	CTCCCAAGGTTGTCGGAATT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.8	58.1	613-719 bp	1.0	81.2
ycf3-trnS-GGA_p5	CTCCCAAGGTTGTCGGAATT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	634-740 bp	1.0	80.6