Hotspot

psbZ-trnG-GCC

LSC · 42204-43637 · 1434 bp

Back to Ceriops

Pi 0.0508

MarkerSeek score 75.70

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1434
variable_sites: 90
pi: 0.0508
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	1376-1711 bp	1.0	87.2
psbZ-trnG-GCC_p2	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	1372-1707 bp	1.0	85.5
psbZ-trnG-GCC_p3	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	1375-1710 bp	1.0	84.4
psbZ-trnG-GCC_p4	TCTCCTGATGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.8	57.8	1374-1709 bp	1.0	80.8
psbZ-trnG-GCC_p5	CTTCTCCTGATGGTTGGTCG	ACCATTCGACTATATCCGCC	55.0	50.0	58.0	57.0	1561 bp	0.333333	44.9