Hotspot

trnC-GCA-petN

LSC · 31108-32186 · 1079 bp

Back to Ceriops

Pi 0.0298
MarkerSeek score 74.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1079
variable_sites
43
pi
0.0298
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG GCAGCCCAAGCGATACTTAT 50.0 50.0 58.1 58.1 1061-1281 bp 1.0 85.4
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG TGCCCCATACTACGAGTGAA 50.0 50.0 58.1 58.1 1108-1328 bp 1.0 85.0
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG ACTTCTGCCCCATACTACGA 50.0 50.0 58.1 57.8 1113-1333 bp 1.0 84.9
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG TTCTGCCCCATACTACGAGT 50.0 50.0 58.1 57.8 1111-1331 bp 1.0 84.9
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG CACTTCTGCCCCATACTACG 50.0 55.0 58.1 57.8 1114-1334 bp 1.0 84.4