Hotspot

trnR-UCU-atpA

LSC · 11632-12274 · 643 bp

Back to Ceriops

Pi 0.133

MarkerSeek score 80.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 643
variable_sites: 103
pi: 0.133
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	GCGAGTTGCTCAAACAATCC	50.0	50.0	58.0	58.0	1374-1762 bp	1.0	91.5
trnR-UCU-atpA_p2	CCTAGCCTTCCAAGCTAACG	GCGAGTTGCTCAAACAATCC	55.0	50.0	58.1	58.0	1377-1765 bp	1.0	91.3
trnR-UCU-atpA_p3	CCCCTCTCTCTCGTGTCTAG	GCGAGTTGCTCAAACAATCC	60.0	50.0	58.0	58.0	1603-1992 bp	1.0	90.5
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	CTTTCTAAACAAGCGCAGGC	50.0	50.0	58.0	58.0	1837-2225 bp	1.0	90.0
trnR-UCU-atpA_p5	CCCCTCTCTCTCGTGTCTAG	CTTTCTAAACAAGCGCAGGC	60.0	50.0	58.0	58.0	2066-2455 bp	1.0	89.3