Hotspot

trnT-CGU-trnR-UCU

LSC · 11219-11559 · 341 bp

Back to Ceriops

Pi 0.0993

MarkerSeek score 84.10

Primer available yes

Label trnT-CGU-trnR-UCU

Feature Metadata

label_name: trnT-CGU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 46
pi: 0.0993
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-CGU-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	423-919 bp	1.0	73.3
trnT-CGU-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	426-922 bp	1.0	73.0
trnT-CGU-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	429-925 bp	1.0	72.6
trnT-CGU-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	432-928 bp	1.0	72.3
trnT-CGU-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.7	56.9	427-923 bp	1.0	70.8