Hotspot

trnS-GCU-trnT-CGU

LSC · 8358-10386 · 2029 bp

Back to Ceriops

Pi 0.0779
MarkerSeek score 82.10
Primer available yes
Label trnS-GCU-trnT-CGU

Feature Metadata

label_name
trnS-GCU-trnT-CGU
feature_type
igs
region
LSC
length_bp
2029
variable_sites
176
pi
0.0779
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnT-CGU_p1 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 1669-2493 bp 1.0 80.8
trnS-GCU-trnT-CGU_p2 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 1668-2492 bp 1.0 80.8
trnS-GCU-trnT-CGU_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 1669-2493 bp 1.0 80.8
trnS-GCU-trnT-CGU_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 1668-2492 bp 1.0 80.7
trnS-GCU-trnT-CGU_p5 AGAACCGGAAAGAGAGGGAT ACGAATCGCACTTTTACCACT 50.0 42.9 57.7 58.2 1727-2551 bp 1.0 78.6