Hotspot

trnQ-UUG-psbK

LSC · 6747-7336 · 590 bp

Back to Ceriops

Pi 0.0654

MarkerSeek score 83.70

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 45
pi: 0.0654
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	ATAGGTATGCTCTGGGACGG	TAGGCTTCGGGCAATTTTGT	55.0	45.0	58.4	58.1	627-770 bp	1.0	85.4
trnQ-UUG-psbK_p2	ATAGGTATGCTCTGGGACGG	GCTTCGGGCAATTTTGTGAA	55.0	45.0	58.4	57.9	624-767 bp	1.0	84.9
trnQ-UUG-psbK_p3	ATAGGTATGCTCTGGGACGG	GTAGGCTTCGGGCAATTTTG	55.0	50.0	58.4	57.7	628-771 bp	1.0	83.8
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	TAGGCTTCGGGCAATTTTGT	50.0	45.0	58.7	58.1	582-725 bp	1.0	83.2
trnQ-UUG-psbK_p5	GACCAAAACCCGTTGCCTTA	GCTTCGGGCAATTTTGTGAA	50.0	45.0	58.7	57.9	579-722 bp	1.0	82.7