Hotspot

ycf1

SSC · 127162-132825 · 5664 bp

Back to Cercocarpus

Pi 0.00165

MarkerSeek score 41.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5664
variable_sites: 14
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCCCTCGGATTCTTTTTCCT	GAACTTTGGTTCTTCCCAGA	50.0	45.0	57.9	55.2	231 bp	1.0	57.1
ycf1_p2	CTTGGCCCTCGGATTCTTTT	GAACTTTGGTTCTTCCCAGA	50.0	45.0	58.2	55.2	235 bp	1.0	56.8
ycf1_p3	TGGCCCTCGGATTCTTTTTC	GAACTTTGGTTCTTCCCAGA	50.0	45.0	58.2	55.2	233 bp	1.0	56.8
ycf1_p4	TTGGCCCTCGGATTCTTTTT	GAACTTTGGTTCTTCCCAGA	45.0	45.0	57.7	55.2	234 bp	1.0	55.7
ycf1_p5	GCCCTCGGATTCTTTTTCCT	GAACTTTGGTTCTTCCCAGAA	50.0	42.9	57.9	56.0	231 bp	1.0	55.6