Hotspot

petD

LSC · 79198-80403 · 1206 bp

Back to Cercocarpus

Pi 0.00221

MarkerSeek score 44.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1206
variable_sites: 4
pi: 0.00221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGCGAATTCTCCGA	ACTTTGAAGCGACTATTCCCT	45.0	42.9	57.3	57.0	1309 bp	1.0	58.4
petD_p2	AGTTGAAGCGAATTCTCCGA	CACTTTGAAGCGACTATTCCC	45.0	47.6	57.3	57.0	1310 bp	1.0	58.1
petD_p3	AGTTGAAGCGAATTCTCCGA	GGGAAAATTCACTTTGAAGCGA	45.0	40.9	57.3	57.8	1319 bp	1.0	56.4
petD_p4	AGTTGAAGCGAATTCTCCGA	AGGGAAAATTCACTTTGAAGCG	45.0	40.9	57.3	57.8	1320 bp	1.0	56.3
petD_p5	TGAAGCGAATTCTCCGAAGA	ACTTTGAAGCGACTATTCCCT	45.0	42.9	57.0	57.0	1306 bp	1.0	56.1