Hotspot

clpP-psbB

LSC · 74624-75086 · 463 bp

Back to Cercocarpus

Pi 0.00432

MarkerSeek score 46.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 463
variable_sites: 3
pi: 0.00432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	AAGAGAACGGGGGAATACCA	TCAAACGACCGGGATCATTC	50.0	50.0	58.0	58.0	641 bp	1.0	79.9
clpP-psbB_p2	AGAGAACGGGGGAATACCAT	TCAAACGACCGGGATCATTC	50.0	50.0	57.8	58.0	640 bp	1.0	78.7
clpP-psbB_p3	AAGAGAACGGGGGAATACCA	CAGAAATCAAACGACCGGGA	50.0	50.0	58.0	58.2	647 bp	1.0	78.5
clpP-psbB_p4	AAGAGAACGGGGGAATACCA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	603 bp	1.0	78.2
clpP-psbB_p5	AAGAGAACGGGGGAATACCA	CAAACGACCGGGATCATTCA	50.0	50.0	58.0	58.3	640 bp	1.0	77.9