Hotspot

trnM-CAU-atpE

LSC · 54530-54803 · 274 bp

Back to Cercocarpus

Pi 0.00243

MarkerSeek score 56.10

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 274
variable_sites: 1
pi: 0.00243
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	368-373 bp	1.0	77.8
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.4	58.1	360-365 bp	1.0	77.5
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	50.0	55.0	58.4	58.3	367-372 bp	1.0	75.9
trnM-CAU-atpE_p4	GTATTGCTTTCATACGGCGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.3	58.0	369-374 bp	1.0	75.7
trnM-CAU-atpE_p5	GTATTGCTTTCATACGGCGG	CGAGCTAGGACACGAGTAGA	50.0	55.0	57.3	58.1	361-366 bp	1.0	75.5