Hotspot

trnT-GGU-psbD

LSC · 33143-34642 · 1500 bp

Back to Cercocarpus

Pi 0.00133

MarkerSeek score 49.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1500
variable_sites: 3
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	55.0	50.0	58.1	58.0	1627-1664 bp	1.0	81.3
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.1	58.0	1630-1667 bp	1.0	81.2
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	55.0	50.0	58.1	58.0	1629-1666 bp	1.0	81.2
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.1	58.0	1632-1669 bp	1.0	81.0
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.3	58.0	1604-1641 bp	1.0	79.6