Hotspot

petN-psbM

LSC · 29652-30518 · 867 bp

Back to Cercocarpus

Pi 0.00231

MarkerSeek score 61.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 3
pi: 0.00231
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	50.0	45.0	58.0	57.6	964-973 bp	1.0	77.3
petN-psbM_p2	ACATTTTCCCTTTCGCTCGT	AAAACAGTCAGCCAAGGTGA	45.0	45.0	58.1	57.6	927-936 bp	1.0	76.7
petN-psbM_p3	CCCTTTCGCTCGTAGTATGG	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.9	57.6	920-929 bp	1.0	76.5
petN-psbM_p4	CCTTTCGCTCGTAGTATGGG	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.9	57.6	919-928 bp	1.0	76.5
petN-psbM_p5	CTTTCGCTCGTAGTATGGGG	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.9	57.6	918-927 bp	1.0	76.5