Hotspot

trnC-GCA-petN

LSC · 28798-29561 · 764 bp

Back to Cercocarpus

Pi 0.00175

MarkerSeek score 46.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 2
pi: 0.00175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	CAGCCCAAGCGAGATTTACT	45.0	50.0	57.9	58.0	877-882 bp	1.0	79.6
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.1	58.0	821-826 bp	1.0	79.4
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	ACGAGCGAAAGGGAAAATGT	45.0	45.0	57.9	58.1	914-919 bp	1.0	79.0
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	CCATACTACGAGCGAAAGGG	45.0	55.0	57.9	57.9	921-926 bp	1.0	78.7
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	CCCATACTACGAGCGAAAGG	45.0	55.0	57.9	57.9	922-927 bp	1.0	78.7