Hotspot

trnS-GCU-trnG-UCC

LSC · 8472-9032 · 561 bp

Back to Cercocarpus

Pi 0.00357

MarkerSeek score 46.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 3
pi: 0.00357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AATCAAACCGAAAGACCCCT	50.0	45.0	58.0	57.0	671 bp	1.0	72.5
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.0	57.0	670 bp	1.0	72.5
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	AATCAAACCGAAAGACCCCT	50.0	45.0	58.0	57.0	670 bp	1.0	72.5
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.0	57.0	669 bp	1.0	72.5
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.2	57.0	724 bp	1.0	71.3