Hotspot

trnK-UUU-rps16

LSC · 4228-5179 · 952 bp

Back to Cercocarpus

Pi 0.00595

MarkerSeek score 62.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 952
variable_sites: 8
pi: 0.00595
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1083-1087 bp	1.0	79.2
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1082-1086 bp	1.0	79.2
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	1092-1096 bp	1.0	78.3
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	CCTTGAAAAAGGCGCTCAAC	50.0	50.0	58.2	58.2	1087-1091 bp	1.0	77.6
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	1086-1090 bp	1.0	77.6