Hotspot

ndhG-ndhI

SSC · 122934-123408 · 475 bp

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Pi 0.0228
MarkerSeek score 72.40
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
475
variable_sites
16
pi
0.0228
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AAGACCCGATCCCAGAAAGA AATCAAATTGCTTTGGGCCG 50.0 45.0 58.0 57.9 619-686 bp 1.0 81.9
ndhG-ndhI_p2 TCCCAACGAAAAGGCAGAAA AATCAAATTGCTTTGGGCCG 45.0 45.0 57.9 57.9 682-749 bp 1.0 81.5
ndhG-ndhI_p3 AAGACCCGATCCCAGAAAGA TTTGGGCCGTTTACCAATGT 50.0 45.0 58.0 58.3 608-675 bp 1.0 80.4
ndhG-ndhI_p4 TCCCAACGAAAAGGCAGAAA TTTGGGCCGTTTACCAATGT 45.0 45.0 57.9 58.3 671-738 bp 1.0 80.0
ndhG-ndhI_p5 AAGACCCGATCCCAGAAAGA CTTTGGGCCGTTTACCAATG 50.0 50.0 58.0 57.6 609-676 bp 1.0 79.8