Hotspot

ndhD-psaC

SSC · 121089-121340 · 252 bp

Back to Cercis

Pi 0.0265
MarkerSeek score 69.40
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
252
variable_sites
10
pi
0.0265
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 TGGATCGTCCAATTGGAAATGA AGAGGACTGTGTCGGTTGTA 40.9 50.0 58.0 58.0 421-422 bp 1.0 65.5
ndhD-psaC_p2 TGGATCGTCCAATTGGAAATGA CCGACGGATTTCTTGAGTGT 40.9 50.0 58.0 57.9 381-382 bp 1.0 65.2
ndhD-psaC_p3 TCCGTCAGTTGGATTAATGGA AGAGGACTGTGTCGGTTGTA 42.9 50.0 56.7 58.0 438-439 bp 1.0 63.6
ndhD-psaC_p4 TCCGTCAGTTGGATTAATGGA CCGACGGATTTCTTGAGTGT 42.9 50.0 56.7 57.9 398-399 bp 1.0 63.2
ndhD-psaC_p5 TGGATCGTCCAATTGGAAATGA AAGAGATGTGAATCCGCCTG 40.9 50.0 58.0 57.7 402-403 bp 1.0 63.1