Hotspot

ndhF-rpl32

SSC · 116552-117583 · 1032 bp

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Pi 0.00454
MarkerSeek score 64.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1032
variable_sites
7
pi
0.00454
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCCGTCGGAAAAAGTA AAAGCTTTCAATGCTGTCCA 45.0 40.0 58.0 56.1 1226-1256 bp 1.0 66.7
ndhF-rpl32_p2 TGTTGCCGTCGGAAAAAGTA AAGCTTTCAATGCTGTCCAA 45.0 40.0 58.0 56.1 1225-1255 bp 1.0 66.7
ndhF-rpl32_p3 TTTGTTGCCGTCGGAAAAAG AAAGCTTTCAATGCTGTCCA 45.0 40.0 57.8 56.1 1228-1258 bp 1.0 65.5
ndhF-rpl32_p4 TTTGTTGCCGTCGGAAAAAG AAGCTTTCAATGCTGTCCAA 45.0 40.0 57.8 56.1 1227-1257 bp 1.0 65.5
ndhF-rpl32_p5 TGTTGCCGTCGGAAAAAGTA ACGAAAAAGCTTTCAATGCTGT 45.0 36.4 58.0 58.0 1231-1261 bp 1.0 65.2