Hotspot

trnN-GUU-ndhF

IRb · 113000-114295 · 1296 bp

Back to Cercis

Pi 0.0401
MarkerSeek score 77.70
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
1296
variable_sites
60
pi
0.0401
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.88
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 CGAACCCTTCCATTCCGAAT CAAATATGTAGGGGGTGGCC 50.0 55.0 58.0 58.0 1324-1518 bp 1.0 87.0
trnN-GUU-ndhF_p2 ACGACCAGTCAGTTAACAGC CAAATATGTAGGGGGTGGCC 50.0 55.0 57.8 58.0 1257-1451 bp 1.0 86.3
trnN-GUU-ndhF_p3 AGGATTCGAACCTACGACCA CAAATATGTAGGGGGTGGCC 50.0 55.0 58.2 58.0 1270-1464 bp 1.0 86.2
trnN-GUU-ndhF_p4 GGATTCGAACCTACGACCAG CAAATATGTAGGGGGTGGCC 55.0 55.0 57.8 58.0 1269-1463 bp 1.0 85.8
trnN-GUU-ndhF_p5 CCCCAAGTAGGATTCGAACC CAAATATGTAGGGGGTGGCC 55.0 55.0 57.7 58.0 1278-1472 bp 1.0 84.8