Hotspot

trnS-GCU-trnR-UCU

LSC · 8025-9922 · 1898 bp

Back to Cercis

Pi 0.0112
MarkerSeek score 68.90
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1898
variable_sites
29
pi
0.0112
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1937-1969 bp 1.0 67.9
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1936-1968 bp 1.0 67.9
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1943-1975 bp 1.0 67.2
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1942-1974 bp 1.0 67.2
trnS-GCU-trnR-UCU_p5 ACGGAAAGAGAGGGATTCGA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.2 56.9 1991-2023 bp 1.0 66.6