Hotspot

trnK-UUU-rps16

LSC · 4298-5053 · 756 bp

Back to Cercis

Pi 0.00533
MarkerSeek score 63.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
756
variable_sites
6
pi
0.00533
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG AAAAAGGCGCTCAACCTACA 50.0 45.0 58.6 58.0 899-914 bp 1.0 76.4
trnK-UUU-rps16_p2 GAGTCGCACTTAAAAGCCGA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.6 58.0 900-915 bp 1.0 76.4
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 45.0 45.0 57.3 58.0 897-912 bp 1.0 75.1
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.6 58.2 904-919 bp 1.0 74.7
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.6 58.2 903-918 bp 1.0 74.7