Hotspot

rpoA

LSC · 102359-103372 · 1014 bp

Back to Cercidiphyllum

Pi 0.00394

MarkerSeek score 61.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 4
pi: 0.00394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GGATTGTGTTAAAGATTCAAAATCCA	CCACATAATGGCTGTAGACCT	30.8	47.6	56.9	57.2	1194-1195 bp	1.0	40.3
rpoA_p2	GGATTGTGTTAAAGATTCAAAATCCA	ACATAATGGCTGTAGACCTCC	30.8	47.6	56.9	56.9	1192-1193 bp	1.0	40.3
rpoA_p3	GGATTGTGTTAAAGATTCAAAATCCA	CACATAATGGCTGTAGACCTCC	30.8	50.0	56.9	58.3	1193-1194 bp	1.0	40.3
rpoA_p4	GGATTGTGTTAAAGATTCAAAATCCA	CCACATAATGGCTGTAGACCTC	30.8	50.0	56.9	58.3	1194-1195 bp	1.0	40.3
rpoA_p5	GGATTGTGTTAAAGATTCAAAATCC	CCACATAATGGCTGTAGACCT	32.0	47.6	55.5	57.2	1194-1195 bp	1.0	40.3