Hotspot

ccsA

SSC · 140019-140978 · 960 bp

Back to Cercidiphyllum

Pi 0.00104

MarkerSeek score 56.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGGCATTGGATCTTCTAA	AAATGGTTATCGCCGGCTTA	50.0	45.0	58.0	57.7	1146-1152 bp	1.0	77.1
ccsA_p2	GGCGGCATTGGATCTTCTAA	AATGGTTATCGCCGGCTTAC	50.0	50.0	58.0	58.4	1145-1151 bp	1.0	76.5
ccsA_p3	GGCGGCATTGGATCTTCTAA	TTGTCAGGCCCTTTCTTCAA	50.0	45.0	58.0	57.3	1086-1092 bp	1.0	73.8
ccsA_p4	GGCGGCATTGGATCTTCTAA	TTTGTCAGGCCCTTTCTTCA	50.0	45.0	58.0	57.3	1087-1093 bp	1.0	73.8
ccsA_p5	GGCGGCATTGGATCTTCTAA	GCCGGCTTACACGAGATTTT	50.0	50.0	58.0	58.9	1135-1141 bp	1.0	72.5