Hotspot

rpl32-trnL-UAG

SSC · 138650-139840 · 1191 bp

Back to Cercidiphyllum

Pi 0.00168
MarkerSeek score 49.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1191
variable_sites
2
pi
0.00168
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTCTTTCTACCGGGAATTCA AGCGTGTCTACCGATTTCAC 42.9 50.0 56.1 58.0 1275-1277 bp 1.0 56.9
rpl32-trnL-UAG_p2 TCTCTTTCTACCGGGAATTCA CAGCGTGTCTACCGATTTCA 42.9 50.0 56.1 58.0 1276-1278 bp 1.0 56.9
rpl32-trnL-UAG_p3 TCTCTTTCTACCGGGAATTCA TTAGAAGATCCAATGCCGCC 42.9 50.0 56.1 58.0 1339-1341 bp 1.0 56.7
rpl32-trnL-UAG_p4 TCTCTTTCTACCGGGAATTCA TCCTAAGAGCAGCGTGTCTA 42.9 50.0 56.1 57.9 1285-1287 bp 1.0 55.9
rpl32-trnL-UAG_p5 TCTCTTTCTACCGGGAATTCA CTAAGAGCAGCGTGTCTACC 42.9 55.0 56.1 57.8 1283-1285 bp 1.0 55.4