Hotspot

petA-psbJ

LSC · 87245-88186 · 942 bp

Back to Cercidiphyllum

Pi 0.00318
MarkerSeek score 60.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
942
variable_sites
3
pi
0.00318
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ACGTGTCCAAGGACTTTTGT ACTGGTATTCCTGTGATCGG 45.0 50.0 57.6 56.7 1129-1130 bp 1.0 66.7
petA-psbJ_p2 ACGTGTCCAAGGACTTTTGT CTGGTATTCCTGTGATCGGT 45.0 50.0 57.6 56.7 1128-1129 bp 1.0 66.7
petA-psbJ_p3 ACGTGTCCAAGGACTTTTGT TGGCTCATATTCAGGATTGGG 45.0 47.6 57.6 57.5 1088-1089 bp 1.0 64.8
petA-psbJ_p4 ACGTGTCCAAGGACTTTTGT GGCTCATATTCAGGATTGGGT 45.0 47.6 57.6 57.5 1087-1088 bp 1.0 64.8
petA-psbJ_p5 ACGTGTCCAAGGACTTTTGT ACTGGTATTCCTGTGATCGGT 45.0 47.6 57.6 58.5 1129-1130 bp 1.0 64.6