Hotspot

ycf4-cemA

LSC · 84527-85372 · 846 bp

Back to Cercidiphyllum

Pi 0.00236

MarkerSeek score 53.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 2
pi: 0.00236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.1	58.1	956 bp	1.0	78.8
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	970 bp	1.0	78.2
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	CGAAGGGGAGTGAATGCTTT	45.0	50.0	58.1	58.2	917 bp	1.0	78.0
ycf4-cemA_p4	AAAGCTGCTGAATTGGCCTA	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	968 bp	1.0	77.8
ycf4-cemA_p5	TTGCGTGTACCCATTGAAGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.7	58.1	944 bp	1.0	77.4