Hotspot

ycf3-trnS-GGA

LSC · 68371-69207 · 837 bp

Back to Cercidiphyllum

Pi 0
MarkerSeek score 60.00
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
837
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 915-926 bp 1.0 71.9
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 936-947 bp 1.0 71.4
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 886-897 bp 1.0 71.1
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 47.6 50.0 58.0 58.4 916-927 bp 1.0 69.5
ycf3-trnS-GGA_p5 TTTATCCGCGATCTAGGCAT CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.6 58.1 913-924 bp 1.0 68.8