Hotspot

rps16-trnQ-UUG

LSC · 26995-28574 · 1580 bp

Back to Cercidiphyllum

Pi 0.00127
MarkerSeek score 59.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1580
variable_sites
2
pi
0.00127
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1647-1653 bp 1.0 78.7
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1658-1664 bp 1.0 78.1
rps16-trnQ-UUG_p3 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1668-1674 bp 1.0 76.5
rps16-trnQ-UUG_p4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1669-1675 bp 1.0 76.4
rps16-trnQ-UUG_p5 ATCCACAGCTGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.4 58.1 1670-1676 bp 1.0 74.7