Hotspot

matK-trnK-UUU

LSC · 24238-24951 · 714 bp

Back to Cercidiphyllum

Pi 0.0014

MarkerSeek score 59.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 714
variable_sites: 1
pi: 0.0014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GGAAGTCATGTTGCCGAGAT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	792-793 bp	1.0	78.6
matK-trnK-UUU_p2	GGAAGTCATGTTGCCGAGAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	791-792 bp	1.0	78.6
matK-trnK-UUU_p3	GGAAGTCATGTTGCCGAGAT	GCGACTGTATCTACACACCC	50.0	55.0	58.0	57.8	854-855 bp	1.0	78.3
matK-trnK-UUU_p4	GGAAGTCATGTTGCCGAGAT	GGGTTGCTAACTCAACGGTA	50.0	50.0	58.0	57.5	805-806 bp	1.0	76.3
matK-trnK-UUU_p5	GGAAGTCATGTTGCCGAGAT	TTCGGGTTGCTAACTCAACG	50.0	50.0	58.0	58.5	808-809 bp	1.0	76.0