Hotspot

accD

LSC · 60681-63356 · 2676 bp

Back to Ceratostigma

Pi 0.0552

MarkerSeek score 74.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 2676
variable_sites: 181
pi: 0.0552
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ACGTTACGGTTGGTTATTTGT	CTTAGTCAGCCTTCGATGCA	38.1	50.0	56.1	57.7	2667-2901 bp	0.666667	49.9
accD_p2	ACGTTACGGTTGGTTATTTGT	ACTTAGTCAGCCTTCGATGC	38.1	50.0	56.1	57.7	2668-2902 bp	0.666667	49.9
accD_p3	ACGTTACGGTTGGTTATTTGT	AGTCAGCCTTCGATGCATTG	38.1	50.0	56.1	58.6	2664-2898 bp	0.666667	47.3
accD_p4	ACGTTACGGTTGGTTATTTGT	GTCAGCCTTCGATGCATTGA	38.1	50.0	56.1	58.6	2663-2897 bp	0.666667	47.3
accD_p5	ACGTTACGGTTGGTTATTTGT	TCAGCCTTCGATGCATTGAC	38.1	50.0	56.1	58.6	2662-2896 bp	0.666667	47.3