Hotspot

ycf2-trnI-CAU

IRa · 164170-164497 · 328 bp

Back to Ceratostigma

Pi 0.00813

MarkerSeek score 50.70

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 328
variable_sites: 4
pi: 0.00813
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	CAGAATTCTTGACCCCTCTCA	ATTGGTGTGCATCCAGTAGG	47.6	50.0	57.0	57.6	502-506 bp	1.0	62.0
ycf2-trnI-CAU_p2	ACTCAGAATTCTTGACCCCTC	ATTGGTGTGCATCCAGTAGG	47.6	50.0	57.0	57.6	505-509 bp	1.0	61.9
ycf2-trnI-CAU_p3	CTCAGAATTCTTGACCCCTCTC	ATTGGTGTGCATCCAGTAGG	50.0	50.0	57.9	57.6	504-508 bp	1.0	60.8
ycf2-trnI-CAU_p4	CAGAATTCTTGACCCCTCTCA	GCCAATTATGAGTTGGGTGC	47.6	50.0	57.0	57.4	464-468 bp	1.0	60.8
ycf2-trnI-CAU_p5	ACTCAGAATTCTTGACCCCTC	GCCAATTATGAGTTGGGTGC	47.6	50.0	57.0	57.4	467-471 bp	1.0	60.8