Hotspot

ccsA-ndhD

SSC · 125956-126182 · 227 bp

Back to Ceratostigma

Pi 0.0413
MarkerSeek score 73.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
227
variable_sites
14
pi
0.0413
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GGATATGCTACTTTGGGGTCA ATCAGTTGATAAGGCGGAAGC 47.6 47.6 57.1 58.4 352-353 bp 1.0 57.1
ccsA-ndhD_p2 TGGATATGCTACTTTGGGGTC ATCAGTTGATAAGGCGGAAGC 47.6 47.6 57.1 58.4 353-354 bp 1.0 57.1
ccsA-ndhD_p3 TGGATATGCTACTTTGGGGT ATCAGTTGATAAGGCGGAAGC 45.0 47.6 55.9 58.4 353-354 bp 1.0 54.9
ccsA-ndhD_p4 GGATATGCTACTTTGGGGTCAA ATCAGTTGATAAGGCGGAAGC 45.5 47.6 57.8 58.4 352-353 bp 1.0 54.6
ccsA-ndhD_p5 TTGGATATGCTACTTTGGGGTC ATCAGTTGATAAGGCGGAAGC 45.5 47.6 57.8 58.4 354-355 bp 1.0 54.6